BIOL2142 Lecture Notes - Lecture 12: Plaque-Forming Unit, Hemagglutination Assay, Red Blood Cell
Document Summary
Used to determine quantity of virus in a sample. You need to already have an idea of what you"re looking for, so you can pick them out ppcr - na of virus can be quantified (amplification) Measurement of an observable effect of the virus hemagglutination assay: determines highest dilution of virus that causes red blood cells to clump together. Compare series of dilutions and compare to red blood cell. Gives relative concentration - it"s a relatively higher/lower concentration of virus in this sample compared to this sample. Plaque assays dilutions of virus preparation made and plated on lawn of host cells number of plaques counted results expressed as plaque-forming units (pfu) plaque forming units (pfu) Does not reflect exact number: some viruses may not have infected, some cells may be infected by 2 viruses. Determine smallest amount of virus needed to cause infection (id0 of death (ld)
