BCMB30004 Lecture Notes - Lecture 6: Alpha Helix, Protein Kinase, Serca

• School: University of Melbourne
• Department: Biochemistry and Molecular Biology
• Course: BCMB30004
• Professor: Professor Cheng

Document Summary

* you need to work against the concentration gradient using calcium pumps. There are two main types of calcium pumps: one resides on the plasma membrane and it is called the plasma membrane. Ca2+ atpase and the other resides in the er/sr and it is called the sarcoplasmic reticulum ca2+ * so, because the calcium pumps are operating against a concentration gradient of calcium, it needs to hydrolyse atp to generate energy. * for the calcium pump to work it requires atp to be hydrolysed and the hydrolysis of atp occurs in two steps within the active site of the calcium pump. Step 1: in the first step there is a key aspartic acid residue in the active site and this becomes phosphorylated forming a phosphor-acetate and this is a phosphoenzyme intermediate. * the phospho-asparate bond is extremely labile (easily altered) and because it is so labile its existence is only transient in the active site of the enzyme.