BCH3031 Lecture Notes - Lecture 13: Noncoding Dna, H3K4Me3, Epistasis

The mutation changes the number of domains in the enzyme, which makes it work more efficiently

The mutation changes the amino acid sequence of the lactase protein

The mutation increases the number of copies of the lactase gene that will be found in your genome

The mutation changes whether the lactase sequence is found in an intron or exon

The mutation affects the expression of the lactase gene

The substrate will increase the reaction rate by binding to the allosteric site

The substrate will increase the reaction rate by competing with the inhibitor for the active site

The reaction rate will not change unless the inhibitor can be removed

The enzyme adjusts its shape so that the substrate, but NOT the competitive inhibitor, can bind

The substrate will bind to the competitive inhibitor and block its ability to bind to the enzyme

Transcription regulators bind to the 5' UTR region of a gene

Regulators bind via complementary base-pairing to certain DNA molecules

Covalent bonds form between the transcription regulator and the atoms of the DNA backbone

Every eukaryotic gene has a different transcription regulator that will bind to the 5' end of the gene

Transcription regulators bind to specific DNA sequences via multiple weak non-covalent interactions

DNA -> RNA -> protein

All cells arise from pre-existing cells

DNA provides the complete instructions to create a cell

The identity of a cell is determined through gene expression patterns

All cells contain the same four basic macromolecules

Using both an activator and repressor enables cells to more accurately determine the amount of lactose available in the environment

Enzymes to digest lactose are only made when energy is low and lactose is available

The activator can override the inhibition of the lac operon by the repressor

The repressor can control the enhancer, while the activator can control the promoter

When neither the lac activator or repressor is present, expression of the lac operon is too high

Phosphorylation and acetylation of DNA affect its ability to be compacted

Changes to the sequence of DNA change whether DNA will wrap around histone proteins

Covalent modifications of histones affect the ability of the transcription initiation complex to form

Histones provide the codon sequences needed for translation to occur

The histone code affects which amino acids will get added to a polypeptide