BMS1062 Lecture Notes - Lecture 4: Guanine, Polyacrylamide Gel Electrophoresis, Thymine
14 Aug 2018
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Lecture 4 : manipulation of dna based on sequence properties. Dna sequence related techniques the sequence of dna refers the linear order of nucleotides read from the 5" end to the 3" end techniques that you learn are : Fragmentation of dna via restriction nucleases detection of specific dna sequences through southern blotting the sequencing of dna and an intro to the human genome project. Dna is enormously long chemically monotonous thus dna must be fragmented into manageable sized pieces for analysis involving a set of sequences. Restriction endonucleases restriction nucleases were discovered by researchers trying to understand how bacteria degraded foreign. Many types of bacteria have restriction/modification systems the recognition sites of restriction enzymes are sequence dependent, though with varying degrees of stringency. With the attached methyl groups, the restriction enzyme effectively cannot bind onto the recognition site. ^ some example of type ii restriction endonucleases. The asterisks denote where methylation would occur to protect the sequence.
