MICR3002 Lecture Notes - Lecture 3: Functional Analysis, Cell Culture, Ascoviridae
Document Summary
Lecture 3 virus regulation by micrornas sirnas vs mirnas. Differences in how they are produced, and their function. There are more than one small rna types that make up rna interferons (rnai: sirna, mirna, pirna (not focused on mainly involved with transposons) mirnas. Almost 30 000 mirnas are known (database) Identified in eukaryotes from unicellular flagellates to multicellular plants (slightly difference mechanisms) and animals but not in bacteria. Post-transcriptional regulatory roles in many cellular pathways. Note: utr: untranslated region upstream or downstream of orf (mirna normally binds here) Note: orf: open reading frame (mirna can still bind here) Primary determinant of target selection: overall binding with mirna across mrna is also important for specificity. Every mirna has a seed region key : always 100% complementation. Single mirnas can regulate many genes: happens at random around the genome, thus, target many mrnas (not a 1:1 relationship)
