IMED1002 Lecture Notes - Lecture 28: Rna Splicing, Northern Blot, Western Blot
12 Jul 2018
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Document Summary
Run mrna on gel, it seperates according to size, us eprobe that is compliment, detect it. Whole exon can be deleted as result of mutations in splice signals: exon skipping. Part of exon can be deleted as a result of mutations in normal splice signals-> cryptic splice site selection (sequences resembling normal splice signals) If its not a multiple of 3 that we remove then it changes reading frame. Crypti(cid:272) spli(cid:272)e site= new one that shouldn"t (cid:271)e there. Abnormal splicing of beta-globin rna in patients with beta thalassemia: different single base mutations cause different splicing defects and disease: Reverse mutation- get wild-type: (from mutant back to wild type) Wild type atgc
