IMED2004 Lecture Notes - Lecture 14: Stem-Cell Therapy, Somatic Cell, Blastocyst
25 Mar 2020
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Document Summary
Stem cells as tools: research and therapeutic applications: Before human embryonic stem cells- there was cloning: cultures of human es cells were described in 1998, before this- scientists were focusing on the potential within the nucleus- creating the tech we know as. Cloning: creating a new animal from an old (adult) cell. Contains all the info it needs: demonstrate that the information is all in the nucleus, nuclear gene activity- transcription factors in the egg, success rate for this procedure is relatively low. Influence of epigenetic modifications: younger cell nuclei have better success rates, high levels of gene misexpression in cloned animals, but useful methods for generated es cells. Induced pluripotent stem cells (ipscs: adult stem cells, present in all adult tissue, required for regeneration, multipotent (many, but not all lineage restricted?, not same level of potency and plasticity. --> ecm stimulation that you cannot get on a different dish.
