BIOL3090 Lecture Notes - Lecture 11: Tata Box, Ribosomal Rna, Nucleoplasm
7 Aug 2018
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Document Summary
Focus on pol ii promoters and polymerase. General transcription factors initiation of transcription. Transcription factors aka gene specific transcription factors, transcription activators, recruitment of remodelling factors pic, structural effects on transcription. Promoters class i - drive production of rrna. Experiments show transcription less efficient when promoter is moved. Tata box if you remove tata box, very little transcription. man false starts. 3 types of promoters for pol i, ii, ii. Three polymerases were originally separated through ion exchange chromatography. Named as they came off the column all have different salt sensitivities. Pol i = rrna, pol ii = mrna, Epitope tagging tag protein with an epitope antibody can tag epitope, see what proteins get pulled down with it on blot. Rpb1 important gets phosphorylated on the ctd. 2 and 3 are the core. (homology to (cid:901), (cid:902), (cid:902)"). Some of these proteins are shared in other. Common subunits 5, 6, 8, 10, 12 all 3 pols.
