PSY3710 Lecture Notes - Lecture 2: Differential Centrifugation, Heptane, Ion Exchange
11 Apr 2018
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Document Summary
Biological macromolecules are isolated from biological tissue and cells based on their physiochemical properties. Subcellular fractions must be characterized by either morphology example em or marker enzymes. Extraction of membrane lipids from cellular membrane (organic solvent extraction and chromatography (tlc, hplc, gc) Hydrophobicity is a measurable property of fatty acids and lipids. Partitioning experiment using 2 solvents, example heptane and water (different polarities require different solvents. Measure partition coefficient: keep in mind the different energy consumption. Calculate free energy change: gtr = -rt ln(keq) Extraction and analysis of membrane lipids is the basis of lipidomics. Lipidomics is the idea of a comprehensive catalogue of lipid composition profiles in cells, tissues, species, during development, ageing and disease. Techniques include: thin layer chromatography, adsorption chromatography, gas- solid chromatography, hpl (high performance liquid chromatography), mass spectrometry. Extraction of membrane proteins from cellular membrane (detergents) Types of membrane and how to extract them. Membranes in water are different they need chaperones to extract them.
