BIOL 2104 Lecture Notes - Lecture 21: Hfr Cell, Genetic Recombination, Insertion Sequence
19 Dec 2015
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Document Summary
Bacteria exhibit immense diversity and have important roles in human health, in the environment, and in industrial applications including recombinant dna technology. General methods to propagate bacteria and identify mutants (selection vs. screen) General attributes of bacterial genomes with focus on the model organism e. coli. Insertion sequence (is) elements dot the genomes of many types of bacteria. Is elements can move to other genomic positions via transposition and can serve as sites of plasmid integration. Plasmids are circular dna molecules separate from the bacterial chromosome. They often carry genes that confer antibiotic resistance (r plasmids) Mechanisms by which bacteria exchange genetic material (transformation, conjugation and transduction) and experiments to distinguish the mechanisms (e. g. u-tube experiment and. General mechanisms of bacterial transformation (natural and artificial) Fertility factor (f) and the two possible states: (1) plasmid state, (2) integrated state (hfr) Different hfr strains can be generated that differ in location and/or orientation of integrated f plasmid.
