MICR 221 Lecture Notes - Lecture 10: Transposable Element, Antimicrobial Resistance, Insertion Sequence

• School: Queen's University
• Department: Microbiology and Immunology
• Course: MICR 221
• Professor: Virginia K Walker

Document Summary

Pathogenesis: rapid spread of resistance to antibiotics. Environmental: biodegradation e. g tol plasmids of pseudomonas sp. Biotechnology: vectors for cloning, overexpression etc e. g puc plasmids. Protein that destroys other bacteria, usually closely related species some are conjugative some carry resistance genes. Transposable elements transposition: the movement of pieces of dna around the genome, or genome to plasmid, plasmid to genome transposable elements (transposons, segments of dna that carry genes for transposition, widespread in bacteria, eucaryotes and archaea. Types of transposable elements insertion sequences (is elements: contain only genes encoding enzymes required for transposition e. g. , transposase (needed for movement around the chromosome) composite transposons, carry genes in addition to those needed for transposition. Bordered by short inverted repeat: goes opposite direction on different sides, short: 20-30 base pairs, specific for that insertion sequence. Composite: can contain antibiotic resistant gene, also has ir, transposase between two ir"s.