BIOL 206 Lecture Notes - Lecture 20: Restriction Fragment Length Polymorphism, Microsatellite, Dna Profiling
16 Apr 2016
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Document Summary
Understand how short tandem repeats (str) & single nucleotide polymorphisms (snps) contribute to genetic variation. Understand how rflp is used in dna profiling. 50% of the human genome has intersperses repetitive sequences. Short segments of dna that repeat a few to thousands of times and are found at specific locations in human dna. Many types of repeat polymorphisms occur on human chromosomes. Each individual will have different number of these repeats. Number of repeats at each location are a random combination of repeats in your parents. For a variation to be considered snp, it must occur in at least 1% of the population. Dna sequence of any two people are 99. 9% identical. Technique used by scientists based on the fact that dna of every individual (except identical twins) is unique. Physical position of an str and associated flanking sequence. Both chromosomes of a homologous pair contain same locus.
