BIOC 2300 Lecture Notes - Lecture 6: Cas9, Crispr, Disulfide
19 Jan 2018
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Document Summary
Crispr: clustered regularly interspaced short palindromic repeats: adaptive immunity in bacteria. There are four different levels of protein structure: 30% of proteins in the body have no structure. Cys-sh: most amino acids have chiral alpha carbon, d and l amino acids are enantiomers, enantiomers: not super-impossible, amino acids in proteins are in the l configuration. For multiple chiral centres, the r, s system must be used (most amino acids are s) Need to be able to recognize the structure of each amino acids, be familiar with abbreviations. This: this provides covalent cross-linking, either within or between polypeptide chains, can be within one chain that folds back on itself or within multiple folding chains. Cellular oxidation and reduction: cysis usually in its sh form in an intracellular (reducing) environment, and usually as s-s outside the cell. In vitro, reducing agents maintain sulfhydryl (reduced) form: glutathione is the biological reducing agent, amino acids play many other roles such as signalling and metabolism.
