BIOC 3400 Lecture Notes - Agarose Gel Electrophoresis, Dalhousie University, Agarose
12 Dec 2019
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Document Summary
Analyzation of purified native and heat-denatured of rna and dna using non-denaturing. Dna and rna are negatively charged molecules, which allows them to move down the agarose gel matrix from the negative cathode to the positive anode. Rna and dna samples were diluted into 0. 1 g/ l and 1. 0 g/ l concentrations and gel electrophoresis was carried on, then a picture of the gel was taken. This experiment was conducted to analyze native and heat-denatured rna and dna using non-denaturing agarose gel electrophoresis. Upon observation of the lanes with varying concentrations, it was concluded that nucleic acids migrate differently under different conditions and are affected by heat. The extra bands could have been due to contaminations and/or errors loading the nucleic acids. The purpose of this experiment was to analyze dna and rna that were native and denatured by heat or by non-denaturing agarose gel electrophoresis.
