BIOL 2030 Lecture Notes - Lecture 23: Bamhi, Ecori, Palindromic Sequence
23 Mar 2016
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Document Summary
At neutral ph, dna is negatively charged at the phosphate groups: will move to a positive (+) electrode in a gel. Need an inner medium (agarose) to prevent all the dna from gathering at the + end. Agarose is a polysaccharide purified from red algea: heat agarose in water and salt will form vertical chain structures. Cool down the agarose solution chains will turn helical helical chains associate and form pores. Dna molecules are separated based on size - fractionation. Use ethidum bromide dye to dye the dna dna will fluoresce. Larger molecules migration through pores in gel is impeded by their size: smaller molecules will move through the gel more rapidly, inverse relationship between mobility of a dna molecule and its molecular weight. Rate of migration of a linear dna molecule is inversely related to the log10 of its mass: based on mobility of a known fragment, can extrapolate the weight of an unknown.
