BIOL 112 Lecture Notes - Lecture 2: Chromosome, Bioinformatics, Exon

• School: McGill University
• Department: Biology
• Course: BIOL 112
• Professor: Jacalyn Vogel

Document Summary

Rather than add new polymerase each round, able to isolate polymerase from thermus aquaticus. Bug that lives at 95 c in yellowstone national park, obviously able to withstand high heats o so then you amplify the region of the chromosome that has the rflp of interest. Then cut the amplified dna with restriction enzymes, see if it cuts. If it cuts you have one large band; if not, two shorter bands. As a result, you know what alleles of the particular rflp are present in that individual. Behave in a mendelian fashion - you can distinguish homozygotes from heterozygotes (both long and short bands) o where do you get rflps from in the first place? trial and error. Sequence the dna of individuals, look for common polymorphisms that change the sequence of restriction sites. Look at people who have family histories of cf, look at their rflps, you can find rflps linked to the cf gene.