BIOL 200 Lecture Notes - Lecture 11: Rna Splicing, Polyadenylation, Intron

• School: McGill University
• Department: Biology
• Course: BIOL 200
• Professor: Kenneth Hastings

Document Summary

Lecture 11 rna splicing and polyadenylation: rna splicing : introduction. Regulates transcriptional activity the more phosphorylated it is, the more it promotes elongation. Ctd of rna pol ii is unfolded and very long in comparison with the globular part. Means it can be a scaffold for many processes. It associates with splicing and polyadenylation factors, thus linking these processes with transcription. E(cid:374)su(cid:396)es that (cid:455)ou do(cid:374)"t ha(cid:448)e primary transcripts getting made floating around looking for splicing factors. No spliced rnas stu(cid:272)k i(cid:374) the (cid:374)u(cid:272)leus (cid:271)e(cid:272)ause the(cid:455) (cid:272)a(cid:374)"t fi(cid:374)d pol(cid:455)ade(cid:374)(cid:455)latio(cid:374) fa(cid:272)to(cid:396)s. First realised from work on hybridization experiments, done in adenovirus and mrna produced from that virus. (transcript so abundant could be isolated). Renaturation experiment was done where mrna was allowed to reattach to the dna. Thinner part is single stranded, thicker part is double stranded (mrna has renatured with dna). In prokaryotes no rna splicing, in eukaryotic cells mrna does not correspond to dna spliced.