BIOL 200 Lecture Notes - Lecture 23: Differential Centrifugation, Dna Replication, Natural Density

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4 Dec 2017
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If you make dna from those labeled nucleotides, the. Only things that are synthesized during the times where the label is presented will be labeled. You cannot label dna only by adding it to a medium with labeled nucleotides. There must me synthesis for things to be labeled. The time where the nucleotides were labeled is called the pulse. Use precursors that are chemically identical to the natural substitute, but its atoms are a detectable isotopic variant. (their nuclei change in the number of neutrons) We can make them and let they decay and detect the radioactivity. C13 which is as stable as carbon 12. C13 cannot be used for a radioactive experiment It has an extra neutron and therefore it is more dense. (this is the labeling used in the meselston-stahl experiment) Using precursors that are chemical derivatives of the natural options.

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