BIOL 200 Lecture Notes - Zinc Finger, Hybridization Probe, Electrophoresis

• School: McGill University
• Department: Biology (Sci)
• Course: BIOL 200
• Professor: Richard Roy

Document Summary

Although the main process of eukaryotic transcription is very similar to that of prokaryotes, the actual factors involved make the mechanism much more complex. Initiation of translation is particularly complex in eukaryotes. Requirement of an rna polymerase, nucleotides, complement bonding, all still applies to eukaryotes. Eukaryotic transcription is limited to two or three organelles. Transcription in the mitochondria and plastids (in plants and algae) have a process mechanically similar to prokaryotes. Transcription in the nucleus is by far the most important and complex. There are three eukaryotic rna polymerases, each differing in gene products and in their sensitivity to the mushroom poison mannitin. Rna polymerase i produces mainly rrna precursors such as 28s, 5. 8s and 18s: pol i is not sensitive to mannitin. Rna polymerase iii is responsible for the transcription of trnas, a splicing factor called u6, small stable rnas and rrnas produced outside the nucleus: pol iii is mildly sensitive to mannitin.