BIOL 200 Lecture 21: 21
Document Summary
Lecture 14 gattaca: genomics and next- generation sequencing. Genomics (cid:120) determining the dna sequence of the genome (cid:120) Identifying the location of genes, commonly referred to as annotation. Since the epstein-barr virus was sequenced in 1984, the dna sequence from thousands of viral, organellar, prokaryotic and eukaryotic genomes have been determined. Model organisms (cid:120) solution with multiple copies of one chromosome (cid:120) mechanical shearing of chromosomes into small fragments (cid:120) how to stitch sequence back together again? (cid:198) look for overlap sequences assembling. Assembled sequences are called contiguous sequences or contigs. Sequence consists of a lot of repetitive dna. A lot of repeats or transposable elements similar to one another. If the(cid:396)e"s o(cid:374)e (cid:374)u(cid:272)leotide diffe(cid:396)e(cid:374)(cid:272)e (cid:271)etween the overlap (cid:396)egio(cid:374) it"s (cid:374)ot used must be 100% identical. If one piece can fit in many different places, it is also not used. Scaffold: other information which allows us to place an order to these contigs.
