BIOL 201 Lecture Notes - Lecture 17: Kymograph, Centriole, Tubulin
11 Apr 2018
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Document Summary
We can stabilize tubulin by constructing it with a non-hydrolysable version of gtp called. If you add green fluorescent tubulin, growth and spontaneous catastrophe events can be observed. It can be directly observed in vitro: take a movie of a tubulin fragment growth and split it frame-by frame into a kymograph , this allows us to see the progression of the fragment over time and measure rates. A series of proteins regulate the microtubule cytoskeleton: growth, collapse, where microtubules are expressed, when microtubules grow/collapse, microtubule polymerases, microtubule depolymerases, +tip proteins. Allows other proteins to target microtubule tips: severing enzymes. Cut microtubules in half: microtubule nucleation factors. Take cells and shift to 4 c microtubules all collapse. Heat back up to 37 c rapid blooming of microtubules from centrosome. We can do this with drugs as well: what is the centrosome. Daughter centriole grows from mother centriole after cell division. Pericentriolar material: high electron-density material formed around centrioles.
