BIOL 201 Lecture Notes - Lecture 30: Mad1, Fish Hook, Securin
23 Apr 2016
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Separation: is it connected to spindle, it is directly in middle. Cdk activity is regulated by availability of cyclins cyclin not shown in this growth but same thing happening for s phase. M phase: align chromosomes, segregate, then separate. If you make mistakes on cell with have no copies and the other will have double. Segregating chromosomes perfect: align between the two pole, attach to two poles for tension, kinetochore and dna are attached to microtubules and are pulled apart to make a signal. Need to degrade cyclin to exit m phase. Proteins are degraded by the proteasome after poly-ubiquitination: unfolding, cleavage, discharge. Apc gets target specificity and activation from binding partners. When its activated it is associated by activator complex. When its active, in conjuction with cdc20 a target protein is recognized by cdc20, drags. Apc over to it, apc has e3 ligase activity and you get poly u so it is recognized by the proteasome and degraded.
