BIOL 202 Lecture Notes - Lecture 7: Southern Blot, Map, Centromere
Document Summary
3 point testcross: example of study in fruit fly. V-vermillion eyes; cv-cross-veinless wings; ct-snipped wing edges trihybrid female (v/v+, cv/cv+, ct/ct+) testcross with tester male (v/v, cv/cv, ct/ct: crossing over can occur between any of the three traits producing 8 possible genotypes. When the traits are unlinked, each genotype has the same proportion in the progeny. If one cross over does not interfere another, then we can calculate the probability of double crossing over. When observed double cross over is less than expected evidence for interference. Southern blot map based approach of genetic disease. Unexpected bots that has been associated with disease suggest mutation in the gene, which is responsible for the disease. Or it can be a gene very close to the actual mutation (the short map distance doesn"t allow recombination) Chromosome maps (multiply by because it is octads: physical map contains repeatable darker stain bands, physical map and recombination map do not scale linearly.