BIOL 202 Lecture Notes - Lecture 8: Bulletin Board System, Heterokaryon, Classical Genetics
31 Jan 2018
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Studying gene interactions in diploid organisms you can"t mutagenize. Often faced with series of mutations which influence phenotype. We need to identify mutations of same genes or another gene: can be mutations of the same gene or mutations of different genes, complementation testing. In more complex organisms, we can"t test by trying to feed the organism supplements. Example: white bluebells (mutant phenotype: you find three white mutants and you want to see if they"re mutants for the same or. Self-pollinate them to create true-breeding individuals to create strain a, b, and different genes. If the genes compliment each other (are not for the same gene), the normal phenotype will arise. If the genes do not compliment each other, you will get the mutant phenotype: conclusion: you crossed two mutants for the same gene. Example 2: testing with neurospora crassa: create organisms with mixtures of nuclei, put together haploid fungi into heterokaryon.
