BIOL 300 Lecture Notes - Lecture 2: Cooperative Binding, Open Reading Frame, Galactose
5 Mar 2018
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It"s the one responsible for bringing the rna polymerase to the right position (on the promoter sequence: the and " subunits polymerize rntps, the subunit is not essential for transcription. It stabilizes the complex and aids in its assembly: there are three channels in rna pol, one where dna comes in, one where dna comes out and one where. Differences in e. coli promoter sequences affect the frequency of transcription initiation. 10 location and -35 location: very conserved consensus sequences. 35: ttgact, -10: tataat: by mutating those sequences, you can affect how strong the promoter is. Also sometimes, it is no longer to melt (and open) the dna: only 2 hydrogen bonds between a and t easier to break. Switching the tryptophan genes on and off: operator: a regulatory element in the promoter, transcriptional repressor: negatively regulates gene expression, ligand-induced conformational changes alter affinity of many repressors for.
