BIOL 300 Lecture Notes - Lecture 23: Karyopherin, Drosha, Northern Blot
Document Summary
Lin-14 is a transcription factor that specifies/expresses early events. Lin-28: transcription factor that represses late events: later on during the development, lin-4 suppresses lin-14 no more early events. At the same time lin-4 suppresses lin-23 late events can happen: mutation in lin-14, cannot express early events, a couple of years later they tried to clone these genes, very successful with lin- When you inject double stranded unc-22 rna, the signal disappears the mrna is gone: 2. Important because it tests whether the injection of rna on its own causes a phenotype (whether you need the nucleotide specificity: here you get no phenotype, 3. Off-target has to be avoided: what is relevant for the mechanism are only 20 nucleotides and it"s very easy to have 20 nucleotides that are identical between two different genes. Mechanism: two starting points: the mirna precursor is a gene that occurs in our body and is transcribed.