ANAT 212 Lecture Notes - Lecture 9: Transmembrane Protein, Signal Transducing Adaptor Protein, Calnexin
30 Mar 2020
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Compare: the transmembrane e3 ligases compare to the e3 ligase in the cytosol. How is substrate bound? thioredoxins (pdi, erp57) calnexin. Hrd1 and gp78, chip, scf complexes, n-end rule e3 ligases. Hrd1 and gp78 are transmembrane (multiple transmembrane helices). Whereas the normal e3 ligases tend to be unit by substrate selection as part of the complex. The details of the examples are not on the exam. This is just to illustrate the mechanisms that we already seen in this lecture. Mutation in the gene that code for cftr gives the cystic fibrosis disease. One n linked glycan motif (asn-x-thr/ser) 1 motif in cftr get glycosylated. Translocation: the 1st tm helix act as a signal anchor. Once that gets integrated into the membrane, during translation of cftr, the orientation of that signal anchor is determined by the charges on each side. It turns out that when the first one is inserted, the next 6 tm stitch into place.
