ANAT 565 Lecture Notes - Lecture 7: Small Interfering Rna, Wilson Disease Protein, Atp7A
3 Sep 2016
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Document Summary
Lumenal cargo binding domain bind soluble proteins. Receptor can also be at cell surface, target proteins for degradation. Cytosolic tail w/motifs for trafficking b/w golgi to endosome to lysosome. Mutation in catd neurodegenerative disease in children. Each have b subunit (large), mu (medium), sigma (small) Divergent in other large subunit: y, a, d, e. B are pretty homologous, especially b1 & b2. Evidence that b1 can participate in ap2 formation & vice versa. Core: part of b1 & y, full u1 & s1. Appendages of y1 & b1 - bind clathrin other accessory proteins. Ap1 at golgi, bind cytosolic tail of receptor, assist in formation of ccv, enable receptor traffic to endosome. B appendage w/clathrin y appendage bind diff accessory proteins affecting timing & size of ccv. Mu1 bind tyrosine signals y & s1 complex interact w/dileucine motifs. Yeast 2 hybrid: yeast only grow when 2 proteins are interacting u3 subunit bind motif in lamp2 (tyrosine motif) (see yeast growth)
