BIOL 300 Lecture Notes - Lecture 25: Notch Signaling Pathway, Cytosol, Protein Subunit
5 Mar 2018
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Document Summary
In antibody-affinity chromatography, if you have a protein you want to purify: you fuse the protein with a short 10 amino acid long tag. This tag is the epitope for these types of antibodies. in this case you couple antibodies to the beads: a common tag is the flag-tag to which the. Ligand-receptor binding: non-covalent interaction: molecular complementarity, it"s reversible, ligand + receptor ligand-receptor complex, this occurs with a certain on rate and because it is reversible, there is also a certain off rate . [lr] where kd is the dissociation constant and k d. On: from the dissociation constant you can learn a lot of things: The affinity of interaction between ligand and receptor (how tightly does the ligand bind to the receptor). If the binding is very tight, then the dissociation constant is a very small number (because [lr] is a large number and [r][l] is a very small number: 2.
