BIOC 311 Lecture Notes - Lecture 14: Uncoupling Protein, Atp Synthase, Chemical Energy

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So far, the oxidative phase pumps hydrogen into the ims. Phosphorylation is driven by this proton gradient, is used by complex 5 (atp synthase) The free energy released during electron transport is conserved pumping protons from the mitochondrial matrix to the intermembrane space. The electrochemical gradient thus formed drives atp synthesis when protons flow back into the mitochondria (use chemical potential energy to create chemical energy) No atp produced if you rupture the imm and neutralized the h+ gradient. Imm is impermeable to ions (h+, oh-, k+, cl-) whose movement would discharge the gradient. If you rupture: o2 and reduction will still be used/occur, but not atp. Electron transport is associated with h+ transport out of intact mitochondria. This creates a measurable electrochemical gradient across the imm, a membrane potential, : this membrane potential is measured in v or mv (in the case of mitochondria) Normally, the electron transport chain is coupled (linked to) atp synthesis.

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