BIOC 311 Lecture Notes - Lecture 13: Pyruvate Dehydrogenase, Malic Acid, Durango
18 Jun 2016
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Document Summary
Carboxymethyl carbons of the pro-s arm of citrate always come acetyl-coa. They are not oxidized to co2 during first turn of cac. (recall: even though citrate has 2 carboxymethyl arms, they are distinguishable. Coo- and ch3 of acetate become randomized on reaching succinate. Each succinyl carboxyl contains 1/2 coo- from acetate. These end up as c1 and c4 of oxaloacetate and removed as co2 in second turn. The methyl carbon of acetate which ends up distributing between c2 and c3 of succinate get oxidized to co2 beginning in third round of cac and continuing in subsequent rounds, a smaller percentage each time. Need to be able to know the fate of 3 different carbons: (1) c4 of oxaloacetate (*): ends up on pro-r-arm of citrate and will be released as. Recover all labelling in 1st turn of cac (2) carboxyl carbon of acetate unit (cross): 1st turn of cac: succinate is a completely symmetric molecule, so half the pool of.
