EDKP 395 Lecture Notes - Lecture 3: Malate Dehydrogenase, Succinate Dehydrogenase, Acetyl-Coa

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Kreb Cycle 10/20/2012 7:43:00 AM
AKA citric acid cycle
Invented by Hans Krebs
o Rejected the first 2x he presented his theory
Main function is to remove hydrogen and associated energy bond
b/w hydrogen and various substrates involved in kreb cycle
o H+ contain the PE in food molecules
Enzymes found in mitochondrial matrix citrate synthase enzyme
o Electron transport is found in cortex
OCCURS in mitochondria
Occurs ANAEROBICALLY but the formation of ATP from the kreb
cycle requires the use of O2 O2 Is the last electron acceptor of
kreb cycle (i.e. before ETC)
o This makes is an aerobic method of ATP production
i.e. oxidative phosphorylation
Process
o Pyruvic acid (3C) is converted to acetyl-CoA (2C)
CO2 is produced
o Acetyl-CoA combines with oxaloacetate (4C) to form citrate
(6C)
Enzyme citrate synthase catalyzes this event
o Citrate is metabolized into oxaloacetate
Two CO2 molecules produced
cyclic procedure due to reformation of oxaloacetate
Products during EACH kreb cycle
o 3 molecules of NADH
contributes the most amount of energy
2.5 ATP’s formed
formed at 3 different places
isocitrate isocitrate dehydrogenase alpha
ketoglutarate
alpha ketoglutarate alpha keto. Dehydrogenase
succinyl-CoA
malate malate dehydrogenase oxaloacetate
o 1 molecule FADH2
contribute 2nd most amount of energy
1.5 ATP’s formed for 1 FADH molecule
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NADH >>> FADH2 in terms of richness of
energy (how many ATP’s form from 1
molecule)
Formed during 1 step
Succinate succ. Dehydro. fumarate
o 1 molecule GTP formation is called “substrate-level
phosphorylation”
high energy compound
transfer terminal phosphate group to ADP to form ATP
By product is 3 molecules of CO2
Note: if talking about products produced from kreb cycle AFTER glycolysis,
remember that for every 1 glycolysis there are 2 kreb cycles
Image depicts the 3 products that are formed during kreb cycle
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Oxidative Phosphorylation is required to occur first in order to produce
Acetyl-CoA
3 stages of oxidative phosphorylation
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