ENVB 437 Lecture 22: Eukaryotic cells and viruses – lecture 22 (April 4th 2018)
4 Jun 2018
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Eukaryotic cells and viruses – lecture 20 (April 4th, 2018)
- WHEN things get dysregulated in a cell, transformation of cancers
o The microRNA genome is dysregulated
▪ This dysregulation contributes to the dyregulation of the cell
Slide 74
- The miRNA genes are regulating between 5-12 different transcripts
- you can have an imperfect amtch and affect the transcription in different ways
o so somewhere between 20-50% of our genome is being regulated by these unregulated transcripts and
many of the targets are transcription actors
▪ so you are regulating the regulators
Slide 75, 76
- lin-4 associated with aging in C. elegans
o it is affecting lin-14 which is producing a protein that is an inhibitor of another protein
▪ when we have high levels of the lin-4 miRNA, that will repress that translation of Lin14
• which is an inhibitor of DAF16. And thus C. elegans has a longer life
o vice versa
- the same miRNA is conserved amongst many species including humans
Slide 78
- there arelots of different cancers (Kidner, breast) that show specific members of the let
o you are altering the normal expression of the miRNAs
Slide 79
- because they don’t need full complimentarity they can target multiple messenger RNAs
o miR-1 can intereact with that target (or 2 others)
▪ another miRNA can target those exact same gebes
• these can all interact simultaneously
o you can have miR-1,2,3 or 2,4,5
▪ different combinations of miRNA will affect how the transcript will
be translated
▪ you will end up with different protein products than gene a, b, and c
- they can also interact with the gene product as well (gene transcript)
o very complicated interrelationships amonst the products
slide 80
- many diseases associated with changes in miRNA
o associations with the development of acute leukemia and other
Slide 81
Document Summary
Eukaryotic cells and viruses lecture 20 (april 4th, 2018) When things get dysregulated in a cell, transformation of cancers: the microrna genome is dysregulated, this dysregulation contributes to the dyregulation of the cell. And thus c. elegans has a longer life the same mirna is conserved amongst many species including humans: vice versa. Slide 78 there arelots of different cancers (kidner, breast) that show specific members of the let: you are altering the normal expression of the mirnas. Many diseases associated with changes in mirna: associations with the development of acute leukemia and other. Slide 81 stem cell like properties, ecm modification and proliferation at different sites: all involve changes in the mirna that are being expressed in those cells. Mirna are associating with the 3" untranslated region if you use the first vs. second. Vs. third polyadenylation site you have a different length ltr: which affects how the translation occurs.
