MIMM 413 Lecture Notes - Lecture 5: 18 Months, Splenomegaly, Ergosterol
14 Feb 2013
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Elimination of vl case down to 1 in 10,000. Imiquimod: synthetic molecules, which activate tlr7/8 receptors mediate a th1 response against. Th1 response macrophage activation in killing the parasite. Activation of tlr 7/8 leads to th1 response (il-12) Th2 response survival of the parasite. R848 /imiquimod vaccine adjuvant experiment aka tlr vaccine adjuvant. Balb/c were immunized with: control pbs, autoclaved leishmania major (alm) ag (no adjuvant) just kill the parasite with autoclaving, alm subcutaneously plus topical imiquimod (footpad) Balb/c mice were challenged with l. major subcutaneously in the opposite footpad 2 weeks after immunization. Measure protection with the swelling of the footpad. Challenge infections in vaccinated balb/c mice: adjuvant effect of imiquimod/r848. Imq 1st and then alm = much fewer parasite (thinner footpad) Conclusion: tlr vaccine adjuvant gives better immunological protection. Ifny production from vaccinated mice challenged with l. major ags. Therefore topical imq + alm provides a better ifny response, which is needed for protection.
