PHAR 505 Lecture Notes - Lecture 14: Peptide Mass Fingerprinting, Molecular Replacement, Mass Spectrum
18 Nov 2017
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Prof doesn"t expect us to understand the theory behind phasing in xrc. The main reason why he taught us this is bc he wanted us to know how to get a structure. We only ever measure intensity on a detector: more photons = more intensity. Patterson function: you can use this method to figure out the position of heavy atoms in your structure. If you know this position, you can back calculate the phase of each and every spot phase of your native crystal: these are called phase distributions because we don"t know the exact phase. If you know both phase and intensity you will get the right animal (remember the cat and duck example) Anomalous scattering: very similar but you just have 1 data set with a distorted symmetry, you compare one half of the data set with the other.
