PHAR 562 Lecture Notes - Lecture 10: Schaffer Collateral, Glutamate Decarboxylase, Dentate Gyrus
12 Feb 2020
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Monday, october 7th, 2019 lecture 14: epilepsy, part ii. Review: before 1997, we didn"t know what was happening in the brain after an injury that leads to epilepsy, until in-vitro organotypic hippocampal preparation: Cells are very well characterized and pathways/connectivity are distinct. Structure: dentate gyrus, ca3, ca1, ca3 cell will form connections to each other (only about 20% of them, though) The pyramidal cells are excitable cells that are located in ca1 and ca3, lined up in a lamina structure. In-vivo is like a black box, too complicated as you could damage other regions, have compensation, etc: dissociate cells: doesn"t work in epilepsy as epilepsy requires a network, chose to go in vitro: Remove a piece of hippocampus place it in organotypic culture on a slide. Aka create a hippocampus organoid of sorts. Make a schaffer collateral lesion (schaffer collateral pathway: ca3 cells communicate with ca1 cells through very few connections)
