PHGY 425 Lecture Notes - Lecture 12: Band-Pass Filter, Binomial Distribution, Cauchy Distribution
23 Jan 2018
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History katz and miledi studied release of neurotransmitter without any stimulation. Time constant of the membrane determine channel properties. Voltage clamp control system, you provide a desired input and you compare. Squid axon insert a wire inside (intracellular electrode), extracellular electrode and ground electrode are required to compare synaptic currents: the current that you measure is a measure of the conductance, you provide a step-wise change in potential. You have a lipid bilayer, and proteins imbedded in it. Aqueous pore and a gate depending on the stimulus. Inside out create a seal, pull the electrode and the patch of membrane that was facing the intracellular side of the cell is now facing the extracellular solution. Whole cell patch rupture the membrane and you have an equilibrium between the intracellular solution and the electrode filled with extracellular solution. Time before patch clamp membrane potential and current noise.
