ANTHROP 2D03 Lecture Notes - Lecture 3: Dna Extraction, Hair Follicle, Genetic Testing
26 Sep 2016
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Document Summary
Can be extracted from almost any human tissue. Buccal cells from inside the cheek are used for paternity tests. Sources of dna at crime a scene: blood, semen, hair follicle, saliva. Dna extraction from evidence is compared to dna from known individuals. Spinning dna with wash buffers causes it to stick to the walls of a test tube, extracting it from non-dna materials. Essentially copies / duplicates the given sequence of dna. Changes the temperature in the test tube. Several components happening in a single reaction. Developer found out that it is possible to create a speci c reaction at hot temperatures (100 degrees) which multiples the dna sequence. At 55 degrees c the sequence is found, then at 72 degrees c it undergoes the process of extension. Nothing in chemistry of biology that compares to this process. All done by enzymes in a tube. A way to target a very speci c sequence in dna/genome.
