BIOLOGY 1A03 Lecture Notes - Lecture 27: Microbiological Culture, Splenda

• School: McMaster University
• Department: Biology
• Course: BIOLOGY 1A03
• Professor: Alastair Tracey Rosa Da Silva

Document Summary

Saira kapadia (cid:862)will glucose and sucrose, as dietary additives, decrease the lifespan of wild-type caenorhabditis. Materials: e. coli op50, worm picker, white sugar (glucose, splenda (fructose, wild-type c. elegans, plates with nematode growth media, pipet. Seeded plates stored in an air-tight container will remain usable for 2 3 weeks. Repeat this process every 2 days to prevent contamination with progeny: record the amount of live c. elegans each day. Eggs will be laid at this time: after the original offspring reaches maturity (2 days), transfer 20 of the adults to a new culture.