BIOLOGY 2C03 Lecture : Module 8(1-2) - March15-19 - BIO 2C03.docx
Document Summary
Lecture 1 the dark genome (march 15) Complexity in higher eukaryotes is explained by: higher frequency of alternative splicing, more extensive use of post-translational modifications (phosphorylation, acetylation, methylation, sumoylation, Frequent use of alternative promoters, termination sites etc. Yeast has 6000, the fly has 12000, the nematode has 19000 genes. The mouse genome has a predicted 22,000 genes but only 16,700 transcripts have been cloned. A few percent of the genome (3-10%) is transcribed into rna. Much of the gene discovery effort is evidence-based and relies extensively on the characterization of est libraries. Therefore, the genome is a desert composed mainly of junk dna. More complete analysis of transcripts expressed in mammalian genomes. Driven by post-human genome projects as early as 2003. Dependent on large research consortia such as fatom or encomde. Based on novel technologies (eg/ tiling arrays, deep sequencing analysis and genomic/bioinformatics tools) Global genome transcription a new view of the transcriptome.
