BIOLOGY 1A03 Lecture Notes - Lecture 4: The Sequence, Transfer Rna, Base Pair

• School: McMaster University
• Department: Biology
• Course: BIOLOGY 1A03
• Professor: Rosa Da Silva

Document Summary

Theme 4: module 3 applications of dna replication. Ability to amplify dna in a tube via polymerase chain reaction (pcr) was developed by kary mullis (1985) Allowed scientists to copy millions of copies of. Technique shed light on diagnosis of genetic defects, detection of viral dna, producing large amounts of dna from fossils containing trace amounts of dna, and linking specific individuals to dna samples during forensic investigations. Does not require multiple enzymes to unwind and stabilize the dna heating and cooling. Except a special dna polymerase tolerant to high temperatures is required: ex. Taq polymerase isolated from the bacterial species thermus aquaticus lives in hot springs with temperatures as high as 95 degrees celsius. Three key stages denaturation, annealing, and extension. Primers are designed to bind or anneal to their complementary sequence on either side of the dna sequence of interest on the dna template strands. Denaturation reaction mixture heated to denature (separate) the strands of the double-stranded dna.