BIOLOGY 2A03 Lecture Notes - Lecture 1: Oxidative Phosphorylation, Enzyme Kinetics, Reaction Rate Constant

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All physiological activity required energy in the form of atp. It is the sum of all the chemical reactions of a cell: examples: hydrolysis, condensation, phosphorylation (used to regulate activity), redox, these reactions are all modulated by equilibrium. It is the energy required to go from reactants, through the transition state, and to the products. It can be reduced by a catalyst like and enzyme. Enzyme kinetics: enzymes are not altered by the reaction. They are specific to a substrate and can catalyze forward and reverse reactions: depends on: Concentration of enzyme (since vmax is the product of [e] and kcat) Kcat (or the rate constant, a property of the enzyme and how fast it turns over substrate) The affinity of the enzyme for the substrate. Physical factors like ph and temperature: they can be represented by the michalis-menten equation: Km is where the enzyme performs at of its maximum velocity or vmax: an enzyme can be regulated:

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