BIOLOGY 2B03 Lecture 3: Protein Purification (Module 3)
30 Nov 2016
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When & why researchers need to isolate a protein. Chemical basis for some commonly used purification techniques. Structural analysis- may want to study folded 3-d structure of protein using x- ray crystallography. Functional analysis- may want to identify protein"s function. May want to identify some of amino acid sequence to predict sequence of gene that codes for protein. May want to develop antibody specific to protein. Antibody can then be used in immunofluorescence microscopy or in. Determine purity (via unique assay form step 1) Used to detect a specific protein & estimate its concentration. Example (detection of enzyme): measure enzymatic activity of protein by looking for release of product or use of substrate. Example (immunological assay): if you have an antibody unique to. Example (immunological assay): if you have an antibody unique to protein, then use antibody to monitor presence & concentration of protein.
