BIOLOGY 2B03 Lecture 9: (Module 9) Identifying Cell Cycle Regulators in Yeast
30 Nov 2016
School
Department
Course
Professor
Document Summary
Bio2b03- module 9: identifying cell cycle regulators in yeast. Unbiased search for genes involved in particular mechanism. If interested in identifying genes required for precise regulation cell cycle, start by assuming every gene in genome is potential candidate; Create mutations randomly in every gene (which usually results in one mutation per cell); look at phenotypic effect of mutations (specifically inhibition of cell cycle or rapid cell division) Mutated gene codes for temperature-sensitive (ts) protein (which is a protein that folds at permissive temperature, but misfolds at restrictive temperature) Allows researcher to change temperature & turn on/off protein function. Cell division cycle (cdc) mutants: mutations in cell cycle regulators; can have one of two effects on cell . Cells delayed in g2 & keep growing instead of entering mitosis. Cells transition into mitosis prematurely & so, cells are smaller than normal. Absence of cdc2 protein, cell fails to divide; cdc2 function increased, cell divides too early & too frequently.
