BIOLOGY 2B03 Lecture Notes - Lecture 14: Papaya Ringspot Virus, Agrobacterium Tumefaciens, Playstation Vr
13 Oct 2017
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Chen, g. , ye, c. , huang, j. et al. (2001). Cloning of the papaya ringspot virus (prsv) replicase gene and generation of prsv-resistant papayas through the introduction of the prsv replicase gene. Papaya ringspot virus (psvr) is an infectious disease present within papaya plants decreasing growth and productivity. The article aimed to evaluate the use of viral genes to produce virus resistance in transgenic plants. Replicase-mediated resistance for psvr within transgenic papaya was studied. The hypothesis was that if the replicase (rp) gene was inserted into the dna of papaya plants, then they would confer resistance to psvr. The main conclusion found from the paper was that transgenic papaya plants with the replicase (rp) gene conferred resistance for psvr. The rp gene was inserted into a plant expression vector and transformed into agrobacterium tumefaciens. The transformed a. tumefaciens was then suspended and inserted into the papaya embryo. Pcr and southern blotting was used to analyze.
