BIOLOGY 2B03 Lecture Notes - Lecture 5: Unfolded Protein Response, Golgi Apparatus, Endoplasmic Reticulum
16 Feb 2016
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Document Summary
Site of fatty acid and phospholipid synthesis, carbohydrate metabolism, and where calcium is kept to regulate [calcium] in cytosol. Site of protein modification and formation of vesicles (transport proteins from golgi) co translational transport, Common for proteins secreted from cell or embedded in the membrane / proteins involved with cellular interactions with extracellular matric and receptor ligand recognition. N linked glycosylation: polysaccharide/sugar group to the nh2 of asp residue addition of covalent a o o. Pdi (protein disulphide isomerase) facilitates the formation of disulphide bridges in proteins, and corrects inappropriate disulphide bridges: pdi interacts with 2 cys intermediate (can speed up rate of reaction) = oxidized pdi, oxidized pdi interacts with 1 cys residue and forms a disulphide bond with it, neighbouring cys on protein then creates a disulphide bridge with the cys involved with pdi (intramolecular cysteine bond) protein is oxidized and pdi is reduced.
