BIOLOGY 3P03 Lecture Notes - Lecture 7: Patch Clamp, Asteroid Family, Neuromuscular Junction
5 Feb 2017
School
Department
Course
Professor
Document Summary
Patch clamp recording uses 4 basic configurations: on-cell or cell-attached patch. Easiest to do and most common 95% of patch clamps: whole-cell clamp (including perforated-patch variation, outside-out patch. Note: advantages and disadvantages of each configuration. Particularly, which configuration is appropriate for the type of channel (and its regulation) under study. Begin by forming gigaseal in the cell-attached configuration. Adding a little suction (pulls membrane in and gives a gigaseal) Increase the suction a little more break the little bit of membrane (cid:271)et(cid:449)ee(cid:374) the(cid:373) = i(cid:374) esse(cid:374)(cid:272)e, (cid:455)ou(cid:859)(cid:396)e not connected to the whole inside of the cell. Mi(cid:454) (cid:272)o(cid:374)te(cid:374)ts of the (cid:272)ell (cid:449)ith (cid:449)hat(cid:859)s inside the pipette daunting experience for the cell depending on is in the pipette. After you go whole cell, pull away mechanically from the cell pulls a little bit of membrane with it. Lipid bilayers will spontaneously reseal, and you get the outside-out configuration.
