HTHSCI 1DT3 Lecture Notes - Lecture 16: Cancer Immunotherapy, Cd19, Viral Vector
18 Mar 2016
School
Department
Course
Professor
Document Summary
Different ways to use viruses for cancer immunotherapy: The cancer girl cured by the hiv virus: misleading title. She was cured by t-cells, not hiv; hiv was the form of delivery (hiv integrates a gene (the t-cell receptor gene) into the host"s genome; cells continually express the gene product: t-cell receptor) Can"t directly use hiv; must delete a lot of genes essential for hiv virus replication. 4 separate fragments >>> dna plasmids to separately express the 4 fragments. Transfect fragments into different site of cell"s genome. In emily"s case: targeting b cell leukaemia with t cells genetically modi ed with receptors that target cd19. Source (peripheral blood ~ 0. 001%) >> enrichment (~80%) >> expansion >> pump the blood back into patient. T cell exhausting (expansion from 10^4 to 10^10) Re: which immune cells to use - the more the better vs. the younger the better (proliferative/young = optimal)
