HTHSCI 1LL3 Lecture Notes - Lecture 10: Germ Cell, Ribosomal Rna, Guanine

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TRANSCRIPTION
DĀ§Ā¢D D
Primary Transcript
ā€¢
Transcription :RNA synthesis 1mm aDNA template
-
uses apolymerase that jaw nucleotides to form asingle strand
-
Difference between the sequence in the DNA coding strand is that Utakes places of TmRNA
RNA Polymerase
ā€¢
Polynucleotide strand is synthesized similar to DNA ,in the 5' ā†’ 3 'direction Icopied tom the template strand in the 3' ā†’ s 'direction )
ā€¢
RNA polymerase is able to initiate synthesis without a primer and lacks a separate proofreading exo nuclease
(coding)ā†message in
RNAIDNA Relationship correct
(template)order
ā€¢
language of DNA ā†’RNA ā†’Protein
to builds RNA
ā€¢3nucleotides code for 1amino acid ā†’takes message
/from DNA Into
cytosol to make
Starting
Cod On Protein
RNA Polymerases
ā€¢Bacteria have one RNA polymerase that transcribes all the different types of RNA in the cell
ā€¢
Eukaryotes have an individual RNA polymerase for each of āœ“ RNA ,mRNA and tRNA
-
RNA polymerase Iā†’
produces VRNA Inever transcribed )
Coding Region
-RNA
polymerase 11 ā†’produces mRNA 5'
31
-
RNA polymerase 111 ā†’produces tRNA Inever transcribed )
ā€¢
An
RNA polymerase must be able to recognize the start point and the correct strand to transcribe
ā€¢
Promoter :site of attachment for the RNA polymerases and identities the start point for transcription (expression of the gene :transcript to pnhm)
Anatomy of a Eukayohc Gene
ā€¢
The gene is made up of a promoter and transcribed region
ā€¢The genetic sequence is numbered from Hfor the start site
ā€¢The "
upstream "
or s'
-flanking region is numbered negatively from +1 latter transcription)
-
TATA box Dhere
ā€¢
Eukaryote genes contain huh coding regions called htrons that must be removed ;RNA must 9110 be modified before transport
Promoters
ā€¢
promoters :binding slkslvr Polymerases (upstream)
-
Consist of an AT rich region in prokaryotes +eukayoks and are
"
a's
"
-acting since they are located on the same molecule
-
trans -actna :proteins that bind and enhance or prevent transcription
Opewns
ā€¢Ideft asingle promoter in bacterial cells that
controls
anumber of genet
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produces pdyuslnnk transcript that is translated to several proteins Kcodes for proteins
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e9 .lacopevon allows e. coli to metabolize lactose for food source
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