BIOL 205 Lecture Notes - Lecture 9: T7 Rna Polymerase, Tata-Binding Protein, Antisense Rna

• School: Queen's University
• Department: Biology
• Course: BIOL 205
• Professor: Ian Chin- Sang

Document Summary

Small functional rnas that regulate and protect the eukaryotic genome. Top strand is the template strand, we build from 5" to 3". Is an rna polymerase from the t7 bacteriophage. T7 polymerase is extremely promoter specific and transcribes only dna downstream of a t& promoter (18bp) (taatacgactcactatag) transcription beginning with the 3" g. T7 pol looks for this sequence, and now we can make rna easily. Used in many in vitro transcription reactions. Dna template with t7 promoter, t7 rna pol, ntps (aucg) What we want to be transcribed follows directly the 3" g. Small noncoding rna molecule (~22 nucleotides) found in plants, animals, some viruses. Functions in rna silencing and post transcriptional regulation of gene expression. 22 nucleotide rna has sequences partially complementary to multiple sequences in the 3" utr of the gene that it silences. Destabilize rna through shortening of is polya tail. Inhibit translation of the mrna into proteins -> not translated.